Fig. 4

Effects of circ_0116061 silencing and miR-477 inhibitor on the biological functions of OA chondrocytes. a The transfection efficiency of anti-miR-200b-3p was evaluated by detecting miR-200b-3p expression using qRT-PCR. b–n OA chondrocytes were transfected with si-NC, si-circ_0116061, si-circ_0116061 + anti-NC or si-circ_0116061 + anti-miR-200b-3p. Cell viability, the cell cloning number, and apoptosis rate were determined using CCK8 assay (b), colony formation assay (c), and flow cytometry (d). e–n WB analysis was used to examine the protein levels of Cyclin D1, Bcl2, Bax, Cleaved-casp3, IL-1β, IL-6, IL-1α, and TNFα. **P < 0.01, ***P < 0.001