Fig. 3
From: Overexpression of CRABP2 inhibits dexamethasone-induced apoptosis in human osteoblast cells
The effects of CRABP2 overexpression on DEX-induced apoptosis of osteoblast. Osteoblasts were pretreated with a CRABP2 expression plasmid and/or PI3K inhibitor (LY294002) 12 h before DEX treatment. a Flow cytometry after Annexin-V/PI staining analysis of apoptosis after DEX (10-6 M, 24 h), DEX+ CRABP2 pcDNA3, and DEX+ CRABP2 pcDNA3+LY294002 treatments. b Western blotting was performed to analyze the expression levels of Bax, Bcl-2, cleaved-Caspase-3, Caspase-3, CRABP2 c, PI3K, p-PI3K, AKT, and p-AKT in osteoblast after DEX (10-6 M, 24 h), DEX+ CRABP2 pcDNA3, and DEX+ CRABP2 pcDNA3+LY294002 treatments. *P<0.05, DEX: dexamethasone