Fig. 5

BCL2L12 participated in the miR-24-3p-induced protective effects against IL-1β-induced chondrocyte injury in vitro. Chondrocyte cell line CHON-001 was co-transfected with miR-24-3p mimics and pcDNA3.1-BCL2L12 or pcDNA3.1, followed by IL-1β stimulation for 24 h. a The expression of BCL2L12 was detected by reverse transcription quantitative PCR. b Cell viability was examined by CCK-8 assay. c Caspase-3 activity was analyzed using by commercial Caspase-3 Colorimetric Activity Assay Kit. d The release of TNF-α and IL-18 in supernatant of CHON-001 cells from different groups was determined by ELISA assay. e, f The protein levels of BCL2L12, MMP-13, ADAMTS-5, ACAN, and COL2A1 were measured by Western blot assay. Data were presented as mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001, compared with miR-NC + pcDNA3.1; ##p < 0.01, ###p < 0.001, compared with miR-24-3p mimics + pcDNA3.1