Fig. 3

Validation of RNA-seq data by qRT-PCR. The relative expression data with their corresponding error bars were derived from three technical replicates in an experiment representative of several independent ones. The asterisk *, **, and *** indicate levels of significance of differential expression tested by Student’s t test with p value <0.05, <0.01, and <0.001, respectively. Gene expression level for each gene is calculated as the fold change of the DAE group to the blank group