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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Knockdown of Circ_SLC39A8 protects against the progression of osteoarthritis by regulating miR-591/IRAK3 axis

Fig. 6

Overexpression of IRAK3 partially reversed miR-591 on chondrocyte apoptosis. a Overexpression efficiency of IRAK3 was determined by western blot assay in OA chondrocytes transfected with IRAK3 or vector. b IRAK expression in OA chondrocytes transfected with NC, miR-591, miR-591 + vector, or miR-591 + IRAK3. c Cell apoptosis was determined using flow cytometry analysis. d The protein levels of Bcl-2, Bax, caspase 3, cleaved-caspase 3, caspase 9, and cleaved-caspase 9 were analyzed by western blot assay. e Western blot assay was carried out to examine the protein expression of TNF-α, IL-1β, IL-6, MMP3, and COL2A1. *P<0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X