Fig. 6

The exosomes secreted by mBMSC accelerated M2 macrophage polarization. RAW 264.7 cells were cultured with exosomes secreted by mBMSCs which incubated with WKYMVm peptide (1 μmol/L) or WKYMVm peptide (1 μmol/L) plus WRW4 peptide (10 μmol/L) for 24 h. (a) Morphology of RAW 264.7 cells under optical microscope. DAPI (blue), CD206 (green), and merge (blue/green) were observed by immunofluorescence microscopy in (b) (control group), (c) (WKYMVm peptide group), and (d) (WKYMVm peptide plus WRW4 peptide group). (e) The quantification of M2 macrophage polarization was observed by immunofluorescence (Marker: CD206). (f and g) The quantification of CD206 and Arg-1 protein expression levels which was the makers of M2 macrophage was analyzed by western blotting. Data represent means ± SD. *P < .05, **P < .01, and ***P < .001 relative to controls in which the concentration of WKYMVm peptide was 0 μmol/L