Fig. 6

The effect of miR-650 and CNP overexpression on cell proliferation, inflammatory response, apoptosis, migration, invasion, and EMT. a, b CNP abundance was examined via qRT-PCR and Western blotting in RAFLSs transfected with pcDNA or CNP overexpression vector. CNP expression (c, d), cell proliferation (e), IL-6 and IL-10 levels (f, g), apoptosis (h), caspase3 activity (i), migration (j), invasion (k), and expression of E-cadherin, N-cadherin, and Vimentin (l) were determined in RAFLSs transfected with miR-NC, miR-650 mimic, and miR-650 mimic + pcDNA or CNP overexpression vector by qRT-PCR, Western blotting, CCK-8, and ELISA or Transwell assay, respectively. **P < 0.01, ***P < 0.001