Fig. 2

Treatment of IL-1β-stimulated chondrocytes with SMN. a Addition of 50 μM SMN further increased cell death (LDH release) of injured cells. b Addition of 25 μM SMN partially restored the viability of stimulated chondrocytes. c IL-1β stimulation impaired GAG production (toluidine blue staining), reduced the number of surviving cells (green fluorescence), and resulted in cell senescence (β-gal staining). Supplementation with 50 μM SMN had no substantial effects, whereas the addition of 25 μM SMN ameliorated GAG production, cell survival and β-gal expression in IL-1β-injured chondrocytes