Table 2 PCR conditions for IL-6-174G/C, CALM1-16C/T, and TGFB1-29C/T
From: Genetic markers of osteoarthritis: early diagnosis in susceptible Pakistani population
| Â | IL-6-174G/C | CALM1-16C/T | TGFB1-29C/T | |||
|---|---|---|---|---|---|---|
Temperature/°C | Time | Temperature/°C | Time | Temperature/°C | Time | |
Denaturation | 94 | 03:00 | 95 | 15:00 | 95 | 10:00 |
Denaturation | 94 | 00:30 | 95 | 00:30 | 95 | 00:15 |
Annealing | 57 | 00:50 | 60 | 01:30 | 57 | 00:50 |
Extension | 72 | 00:40 | 72 | 01:30 | 72 | 00:40 |
Final Extension | 72 | 03:00 | 72 | 10:00 | 72 | 05:00 |