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Table 2 PCR conditions for IL-6-174G/C, CALM1-16C/T, and TGFB1-29C/T

From: Genetic markers of osteoarthritis: early diagnosis in susceptible Pakistani population

  IL-6-174G/C CALM1-16C/T TGFB1-29C/T
Temperature/°C Time Temperature/°C Time Temperature/°C Time
Denaturation 94 03:00 95 15:00 95 10:00
Denaturation 94 00:30 95 00:30 95 00:15
Annealing 57 00:50 60 01:30 57 00:50
Extension 72 00:40 72 01:30 72 00:40
Final Extension 72 03:00 72 10:00 72 05:00