Fig. 2

Effect of HSP22-knockdown on the PGF2α-induced release of VEGF in MC3T3-E1 cells. The cells were transfected with 50 nM of HSP22-siRNA (#1 or #2) or negative control-siRNA (Neg) for 24 h and then stimulated by 10 μM of PGF2α or vehicle for another 48 h. VEGF concentrations in the conditioned medium were determined. The values were presented as the levels adjusted for the total protein levels of whole-cell lysates. The data were analyzed by analysis of variance followed by Bonferroni method for multiple comparisons between pairs. The values of p < 0.05 were considered to be statistically significant. Each value represents the mean ± S.E.M. of triplicate determinations from three independent cell preparations. *p < 0.05 compared to the value of negative control-siRNA-transfected cells without PGF2α-stimulation. **p < 0.05 compared to the value of the negative control-siRNA-transfected cells with PGF2α-stimulation