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Fig. 6. | Journal of Orthopaedic Surgery and Research

Fig. 6.

From: Circular RNA circ_0081001 knockdown enhances methotrexate sensitivity in osteosarcoma cells by regulating miR-494-3p/TGM2 axis

Fig. 6.

Knockdown of circ_0081001 enhanced MTX sensitivity through regulating miR-494-3p and TGM2 expression in MTX-resistant OS cells. a The protein abundance of TGM2 was measured using WB analysis in U2OS/R and HOS/R cells after transfection with vector and TGM2. b–n U2OS/R and HOS/R cells were transfected with sh-NC, sh-circ_0081001, sh-circ_0081001 + anti-miR-NC, sh-circ_0081001 + anti-miR-494-3p, sh-circ_0081001 + vector, or sh-circ_0081001 + TGM2. b, c IC50 value for MTX was calculated in MTX-treated U2OS/R and HOS/R cells. d, e Cell apoptosis was measured by flow cytometry. f–i Transwell assay was utilized to test cell migration and invasion capacities. j–n The protein levels of cleaved-casp3, E-cadherin and N-cadherin were determined by WB analysis. **P < 0.01

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X