Fig. 4

TGM2 was a direct target of miR-494-3p in MTX-resistant OS cells. a Starbase showed that miR-494-3p potentially targeted TGM2. b, c Dual-luciferase reporter assay was conducted to test the luciferase activity in U2OS/R and HOS/R cells co-transfected with TGM2 WT or TGM2 MUT and miR-494-3p or miR-NC. d WB assay was performed to analyze the protein expression of TGM2 in U2OS, U2OS/R, HOS, and HOS/R cells. e, f Relative miR-494-3p expression was detected using the qRT-PCR analysis in U2OS/R and HOS/R cells after transfection with miR-NC, miR-494-3p, anti-miR-NC, or anti-miR-494-3p. g, h The protein abundance of TGM2 was assessed by WB analysis in U2OS/R and HOS/R cells after transfection with miR-NC, miR-494-3p, anti-miR-NC, or anti-miR-494-3p. **P < 0.01