Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D

Table 1 Human primers used for the quantitative PCR evaluation

Gene	Forward primer 5ʼ ➔ 3ʼ	Reverse primer 5ʼ ➔ 3ʼ	Accession number
OC	CCAGCGGTGCAGAGTCCAGC	GACACCCTAGACCGGGCCGT	NM-199173.3
OP	CTCCATTGACTCGAACGACTC	CGTCTGTAGCATCAGGGTACTG	NM-000582
ALP	CTGGGCTCCAGGGATAAAGC	TCAGTGTCTCTTGCGCTTGG	NM-000478.4
OPG	CCGGAAACAGTGAATCAACTC	AGGTTAGCATGTCCAATGTG	NM-002546.3
COL1	AGCGGACGCTAACCCCCTCC	CAGACGGGACAGCACTCGCC	NM-000088.3
β-Tub	GAGGGCGAGGACGAGGCTTA	TCTAACAGAGGCAAAACTGAGCACC	NM-001069.2

OC osteocalcin, OP osteopontin, ALP alkaline phosphatase, OPG osteoprotegerin, Col1 collagen type 1α1, β-Tub β-tubulin

ISSN: 1749-799X