Fig. 4

Real-time PCR and Western blot analysis of Bax expression in degenerated NP cells transfected with miR-573 inhibitors following HBO treatment. a Bax mRNA expression was down-regulated after HBO treatment (**p < 0.01; n = 4). Anti-miR-573 partly reversed the suppressive effects of HBO (^※p < 0.05; n = 4). b Bax protein expression was significantly down-regulated after HBO treatment (**p < 0.01; n = 4). MiR-573 inhibitors partly reversed the suppressive effects of HBO (^※p < 0.05; n = 4). Values were normalized against β-actin. WT, wild type; Mut, mutant