Fig. 2

a The mean viability of the 2-year cryopreserved T-LE Allograft (N = 12) compared with fresh articular cartilage (N = 9). Error bars show each group’s standard deviation. The dotted line representing the 70% viability threshold required for successful clinical outcomes with OCA transplantation. b A live/dead (green, live cells; red, dead cells) stain of the T-LE Allograft (× 10) with visible laser perforations after cryopreservation. Scale bar = 100 μm