Fig. 5
From: MicroRNA-497-5p stimulates osteoblast differentiation through HMGA2-mediated JNK signaling pathway
The potentiation of the JNK pathway ameliorates the stimulative role of miR-497-5p mimic on differentiation. miR-497-5p mimic + AZA-1 or miR-497-5p mimic + DMSO was delivered into MC3T3-E1 cells. a JNK pathway activity in transfected MC3T3-E1 cells. b MTT assessment of MC3T3-E1 cell viability. c Flow cytometric analysis for cell apoptosis. d RT-qPCR measurement of Bax, Bcl-2, OCN, and RUNX2 expression. e ALP staining for ALP activity. f ARS staining for mineralization. Data are presented as the mean ± SD of three replicates each. The unpaired t test was used for the comparison between the two groups (b, c, e, f); the two-way ANOVA was applied for the comparison among multiple groups (a, d). #p < 0.05 vs. MC3T3-E1 cells administrated with miR-497-5p mimic + DMSO