Fig. 2
From: MicroRNA-497-5p stimulates osteoblast differentiation through HMGA2-mediated JNK signaling pathway
miR-497-5p promotes the differentiation of MC3T3-E1 cells. miR-497-5p mimic or NC mimic was transfected into MC3T3-E1 cells. a RT-qPCR measurement of miR-497-5p expression in MC3T3-E1 cells after transfection. b MTT assessment of MC3T3-E1 cell viability. c Flow cytometric analysis for cell apoptosis. d RT-qPCR measurement of Bax, Bcl-2, OCN, and RUNX2 expression. e ALP staining for ALP activity. f ARS staining for mineralization. Data are presented as the mean ± SD of three replicates each. The unpaired t test was used for the comparison between the two groups (a–c, e, f); the two-way ANOVA was applied for the comparison among multiple groups (d). *p < 0.05 vs. MC3T3-E1 cells transfected with NC mimic