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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Knockdown of long non-coding RNA LEF1-AS1 attenuates apoptosis and inflammatory injury of microglia cells following spinal cord injury

Fig. 3

lncRNA LEF-AS1 directly targets miR-222-5p. a Starbase (http://starbase.sysu.edu.cn/) revealed that LEF-AS1 contains binding sequences with miR-222-5p. b RNA immunoprecipitation (RIP) assay to assess enrichment of LEF-AS1 and miR-222-5p in microglial cells. c RNA pull-down performed to assess enrichment of LEF-AS1 in microglial cells. d Relative expression of miR-222-5p in si-NC and si-LEF-AS1 groups. e Relative expression of miR-222-5p in sham and SCI groups. f Relative expression of miR-222-5p in control and LPS-treated microglia cells; *P < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X