Table 1 Primer sequences used for qRT-PCR reactions
Gene | Forward primer(5′ to 3′) | Reverse primer(5′ to 3′) |
|---|---|---|
HO-1 | 5′- AAGACTGCGTTCCTGCTCAAC-3′ | 5′- AAAGCCCTACAGCAACTGTCG-3′ |
IL-1β | 5′-TTCAGGCAGGCAGTATCACTC-3′ | 5′-GAAGGTCCACGGGAAAGACAC-3′ |
IL-6 | 5′-CCTGAACCTTCCAAAGATGGC-3′ | 5′-TTCACCAGGCAAGTCTCCTCA-3′ |
IL-8 | 5′-ACTGAGAGTGATTGAGAGTGGAC-3′ | 5′-AACCCTCTGCACCCAGTTTTC-3′ |
MMP-1 | 5′-AAAATTACACGCCAGATTTGCC-3′ | 5′-GGTGTGACATTACTCCAGAGTTG-3′ |
GAPDH | 5′-ATCTCCTTTGTTACCGCTTCC-3′ | 5′-GAAGATGGTGATGGGATTTC-3′ |