Table 1 Oligonucleotide primers used in real-time PCR assay
From: The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes
Gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|
β-catenin | 5′-GAGGAGATGTACATTCAGCAG-3′ | 5′-GTCTCCGACCTGGAAAAC-3′ |
TCF-4 | 5′-CTTTCCCTAGCTCCTTCTTC-3′ | 5′-CTACGATGGAAAGTGGACAT-3′ |
OPN | 5′-GTGGGAAGGACAGTTATGAA-3′ | 5′-CTGACTTTGGAAAGTTCCTG-3′ |
GAPDH | 5′-CAATGACCCCTTCATTGACC-3′ | 5′-GACAAGCTTCCCGTTCTCAG-3′ |