Fig. 5
From: Lupeol inhibits osteosarcoma progression by up-regulation of HMGA2 via regulating miR-212-3p
MiR-212-3p suppressed proliferation, apoptosis, and invasion in osteosarcoma cells by regulating HMGA2 in vitro. MNNG/HOS and MG-63 cells were transfected with miR-NC, miR-212-3p, miR-212-3p + Vector or miR-212-3p + HMGA2. a–c QRT-PCR and western blot were used to evaluate mRNA and protein expression level of HMGA2 separately. d, e Cell viability was detected by the MTT assay. f, g Flow cytometry and transwell invasion assays were used to detect apoptosis rate and the numbers of invaded cells differentially. *P < 0.05, n = 3