Table 1 List of primer sequences were used in the study
Name | Primer | Sequence (5′-3′) |
|---|---|---|
miR-596 | Forward | AAGCCTGCCCGGCTCCT |
Reverse | GCTGTCAACGATACGCTACGT | |
Smad3 | Forward | CCTCTCCAGCAATAATCCGAA |
Reverse | TGCCCAATTTTCTTTACCAGT | |
ALP | Forward | TGACCTTCTCTCCTCCATCC |
Reverse | CTTCCTGGGAGTCTCATCCT | |
OPN | Forward | CCAAGCGTGGAAACACACAGCC |
Reverse | GGCTTTGGAACTCGCCTGACTG | |
Runx-2 | Forward | ACTTCCTGTGCTCGGTGCT |
Reverse | GACGGTTATGGTCAAGGTGAA | |
Osterix | Forward | GGCACAAAGAAGCCGTACTC |
Reverse | GCCTTGTACCAGGAGCCATA | |
U6 | Forward | CGCTTCGGCAGCACATATAC |
Reverse | TTCACGAATTTGCGTGTCAT | |
GAPDH | Forward | TCGACAGTCAGCCGCATCTTCTTT |
Reverse | GCCCAATACGACCAAATCCGTTGA |