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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

Fig. 5

MiR-596 regulated GC-BMSC proliferation and osteogenic differentiation through regulating Smad3. a MTT assay. b ALP staining. c Alizarin red staining. d The mRNA expression of ALP, OPN, Runx-2, and Osterix was determined using qRT-PCR. e The protein level of ALP, OPN, Runx-2, and Osterix was determined using western blotting. **P < 0.01 vs NC inhibitor. ##P < 0.01 vs miR-596 inhibitor

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