Fig. 5From: MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral headMiR-596 regulated GC-BMSC proliferation and osteogenic differentiation through regulating Smad3. a MTT assay. b ALP staining. c Alizarin red staining. d The mRNA expression of ALP, OPN, Runx-2, and Osterix was determined using qRT-PCR. e The protein level of ALP, OPN, Runx-2, and Osterix was determined using western blotting. **P < 0.01 vs NC inhibitor. ##P < 0.01 vs miR-596 inhibitorBack to article page