Fig. 3

MiR-596 regulated GC-BMSC proliferation and osteogenic differentiation. GC-BMSCs were transfected with miR-596 mimics or miR-596 inhibitor. a The proliferation ability of GC-BMSCs was measured using MTT assay. b ALP staining. c Alizarin red staining. d The mRNA expression of ALP, OPN, Runx-2, and Osterix was determined using qRT-PCR. e The protein level of ALP, OPN, Runx-2, and Osterix was determined using western blotting. **P < 0.01 vs NC mimics. ^##P < 0.01 vs NC inhibitor