Fig. 5

AntagomiR-1323 promotes the formation of bone in the rat femoral fracture model. a Schematic overview of the in vivo rat femoral fracture model. AntagomiR-1323 or antagomiR-NC was administered locally in the area of the femur fracture site on days 4, 7, or 11. n = 9 rats per group. b miR-1323 levels in fracture calluses measured by qRT-PCR on day 14. c X-ray imaging of bone formation at the femur fracture sites on days 7 and 14. d Radiographic score assessment on days 7 and 14. e RUNX2, ALP, and Col I mRNA levels in fracture calluses measured by qRT-PCR on day 14. *p < 0.05, **P < 0.01 [antagomiR-NC vs. antagomiR-1323]. b, d, e Data presented as medians ± interquartile ranges (boxes) and absolute ranges (whiskers)