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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway

Fig. 1

Increased expression of URG4 in osteosarcoma tissues and cell lines. a URG4 expression was significantly increased in osteosarcoma tissues than corresponding normal tissues by HE and IHC, respectively (× 200 magnification). b PCR determined URG4 mRNA expression in osteosarcoma cell lines (HOS, MG63, Saos-2, U2OS, and 143B), and hFOB 1.19 was used as control. c Western blot assay determined URG4 protein expression in osteosarcoma cell lines (HOS, MG63, Saos-2, U2OS, and 143B), and hFOB 1.19 was used as control. d The mRNA expression level of the URG4 in HOS and MG63 cell lines following transfection as determined by RT-qPCR. e The protein expression level of URG4 in HOS and MG63 cell lines following transfection as determined by western blot assay. HE: hematoxylin and eosin; IHC: Immunohistochemistry; URG4: upregulated gene 4; Normal: normal tissues; OS: osteosarcoma tissues; K: blank group; NC: negative control. *p < 0.05, **p < 0.01 vs the NC

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X