Fig. 4

Histological evaluations and peptide nucleic acid fluorescent in situ hybridization. a Samples were from the infected and uninfected tibias at 4 weeks. H&E-stained histological sections (upper lane; scale bars: 100 μm). Gram stain samples (middle lane; scale bars: 100 μm). The presence of fluorescent S. aureus was identified with a peptide nucleic acid fluorescent in situ hybridization probe for bacterial 16S rRNA (lower lane; scale bars: 100 μm). b Working model: ASyycG overexpression resulted in the decreased transcription of icaA and icaD. It was revealed that inactivating the yycG gene probably lead to impaired PIA synthesis. The S. aureus biofilm suppressed the invasive ability and pathogenicity in ASyycG mutants