Fig. 1
Correlation of farnesoid X receptor (FXR) and microRNA-23b-3p expression in osteosarcoma (OS) cells and miR-23b-3p specifically targets cyclin G1 (CCNG1). As FXR might regulate the expression of miR-23b-3p in OS cells, we measured the expressions of FXR and miR-23b-3p in normal osteoblasts (hFOB1.19) and five osteosarcoma cell lines (MG-63, HOS, U2OS, SAOS2, SJSA1). a The relative expression levels of FXR were much downregulated in OS cell lines, especially in MG-63 cells. b The expressions of miR-23b-3p were obviously decreased, especially in MG-63 cells compared with hFOB1.19 cells. c Scatter plots showed the correlation of FXR and miR-23b-3p expression in normal osteoblasts and five osteosarcoma cell lines (Pearson’s coefficient test R2 = 1.00, P = 0.0028). d The changes of miR-23b-3p levels were measured under different concentrations of the FXR agonist, GE4064 (0, 0.5, and 5 μM). e TargetScan predicted that the fragment of CCNG1-3′-UTR contained a binding site of miR-23b-3p. f The correlation between miR-23b-3p and CCNG1 was verified by luciferase reporter assay. Each value represents mean ± SEM (n = 3). GAPDH and U6 served as internal controls for cellular genes and miR-23b-3p, respectively. **p < 0.01 vs. hFOB1.19 cells or blank group; ##p < 0.01 vs. 0.5 μM GW4064 group