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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Co-culture of the bone and bone marrow: a novel way to obtain mesenchymal stem cells with enhanced osteogenic ability for fracture healing in SD rats

Fig. 5

a (A-D): The expression levels of ALP and calcium in MSCs from different sources were detected by the modified Gomori Calcium-Cobalt method and alizarin red staining. b: Statistical analysis results. The expression levels of ALP and calcium were obviously higher in B-BM-MSCs than in BM-MSCs (ALP 26.27% ± 1.11% vs 19.08% ± 1.23%, p < 0.05; calcium 43.05% ± 2.63% vs 6.81% ± 0.72%, p < 0.001; ratio of positive area under each high-power field). n = 6. Magnification: × 60. Scale bar: 200 μm. **p < 0.01, ***p < 0.001

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