Fig. 6
From: Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis
![Fig. 6](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13018-019-1307-6/MediaObjects/13018_2019_1307_Fig6_HTML.png)
ICA attenuated pyroptosis and promoted collagen formation in OA rats. The mRNA levels of NLRP3 (a), IL-1β (b), and IL-18 (c) were examined with qRT-PCR in OA rat cartilage tissue. d The expression level of collagen formation and pyroptosis-related protein was analyzed by Western blotting in OA rat cartilage tissue. e Quantitative analysis of protein band gray in d. GAPDH was used as internal control. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05 and **p < 0.01