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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Impairment of circulating endothelial progenitor cells (EPCs) in patients with glucocorticoid-induced avascular necrosis of the femoral head and changes of EPCs after glucocorticoid treatment in vitro

Fig. 3

Effects of GCs on EPCs in vitro. a CCK8 assay showed that medium to low concentrations of GCs did not affect cells proliferation and cell growth was only suppressed under extremely high concentrations (400 μg/ml and 4 mg/ml). b, c GCs impaired the angiogenesis function of EPCs in vitro. b The average diameter of tubules formed by EPCs treated by different concentrations of prednisolone and methylprednisolone. c Representative images of tube formation assay. d Left panel, western blot analysis for CXCR7, Akt, phospho-Akt(P-Akt), GSK-3β, phospho-GSK-3β(P-GSK-3β), and Fyn in EPCs treated with GC (methylprednisolone, 40 μg/ml) or PBS (Ctrl) for 24 h. Right panel, band density analysis. *p < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X