Fig. 6
Exosome characterization and proteomics analysis of exosomal proteins. (a) Representative TEM image of exosomes isolated from young rat cortical bone (scale bar = 100 nm). b Particle size measurement of exosomes by dynamic light scattering. c Western blot analysis of CD9 and CD81 in exosomes. d The most enriched GO terms among the proteins identified in young rat OLCS exosomes. The abscissa represents protein numbers. e Network of the ageing-related GO terms and associated proteins identified in young rat OLCS exosomes. f Network of the bone remodelling-related GO terms and associated proteins identified in young rat OLCS exosomes. The sizes of the yellow nodes are proportional to the numbers of DEGs related to a given GO term