Fig. 2

The protein expression of the BET family members and the effect of siRNAs in SW1353 cells. a The protein expression of Brd2, Brd3, and Brd4 was confirmed by Western blot assay in SW1353 cells and primary human chondrocytes, respectively. PHC, primary human chondrocyte. b–d SW1353 cells were treated with scrambled siRNA or specific siRNA targeting BRD2, BRD3, and BRD4 for 48 h and then treated with or without IL-1β (10 ng/ml) or TNF-α (10 ng/ml) for 6 h. The presence of Brd2, Brd3, and Brd4 proteins in SW1353 cells was confirmed by Western blot assay