Fig. 5

The proteins expression of pro-inflammatory cytokines in the knees of the mice. Relative protein expression levels of inflammatory cytokines in the articular cartilages of the sham-operated or surgically destabilized mice that were treated with GE-P (300 mg/kg/day), GE-L (300 mg/kg/day) and celecoxib (10 mg/kg/day). Expression levels of the IL-1β, TNF-α and β-actin proteins were determined by western blot and quantitated by microcomputer image device (MCID) image analysis. The β-actin levels were evaluated as a loading control, and the data are expressed as the IL-1β/β-actin and TNF-α/β-actin ratios, respectively. Each value represents the mean ± SE of three replicate experiments. *p < 0.01, significant difference when compared with the vehicle control (Sham). ^#p < 0.05, significant differences when compared with the saline-treated (ACLT) group