Skip to main content

Table 4 Population doubling rate of disc cells cultured in different culture media in each passage

From: Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells

PDR

alpha-MEM

DMEM

Ham’s F-12

alpha/F-12

DMEM/alpha

DMEM/F-12

AF

P0

0.6 ± 0.1

0.6 ± 0.1

0.5 ± 0.1

0.5 ± 0.1

0.6 ± 0.1

0.6 ± 0.1

P1

1.1 ± 0.0

1.0 ± 0.2

0.8 ± 0.2

0.9 ± 0.1

1.0 ± 0.1

1.0 ± 0.1

P2

1.1 ± 0.2

1.1 ± 0.0

1.1 ± 0.2

1.1 ± 0.2

1.2 ± 0.1

1.1 ± 0.2

NP

P0

0.6 ± 0.1

0.5 ± 0.0

0.5 ± 0.1

0.4 ± 0.1

0.4 ± 0.1

0.4 ± 0.1

P1

1.0 ± 0.0

1.0 ± 0.1

0.9 ± 0.1

0.9 ± 0.2

1.0 ± 0.0

1.0 ± 0.0

P2

1.0 ± 0.0

1.0 ± 0.1

0.7 ± 0.1

0.8 ± 0.1

0.9 ± 0.1

0.9 ± 0.1

  1. PDR Population doubling rate (per day) in each passage P0, P1, P2; AF annulus fibrosus; NP nucleus pulposus. Data is shown as mean ± SD (AF n = 5, NP n = 3)