Fig. 3
From: Egr-1 increases angiogenesis in cartilage via binding Netrin-1 receptor DCC promoter
The binding of Egr-1 at DCC promoter regulates the angiogenesis. a Egr-1 expression in OA cartilage. Results are expressed as the ratio of Egr-1 and GAPDH (*P = 0.0003 vs. the sham, n = 5 in each group). b DCC expression in Egr-1 siRNA-treated OA cartilage. Results are expressed as the ratio of DCC and GAPDH (*P = 0.0001 vs. the others, #P = 0.0005 vs. OA group and scramble siRNA-treated group, n = 5 in each group). c VEGF expression in Netrin-1 and Egr-1 siRNA-treated cartilage. Results are expressed as the ratio of VEGF and GAPDH (*P = 0.00005 vs. the others, #P = 0.00009 vs. OA group and scramble siRNA-treated group, n = 5 in each group). d EMSA of nuclear protein from cartilage tissue. Binding activity of DCC gene promoter, containing an Egr-1 site, was examined in nuclear protein (*P = 0.0002 vs. the sham, n = 5 in each group)