Fig. 8

Effect of alcohol on primary cultured MSC integrin β1 mRNA and protein expression. Primary rat MSC were cultured in media alone or media plus 50 mM ethanol for 24 h. Cells were harvested and used for a mRNA or b total protein isolation as described. Int β1 mRNA levels were assessed by qRT-PCR as described. Int β1 protein levels were assessed by western blot analysis as described. a mRNA: media vs EtOH p = 0.0021. b Protein: media vs EtOH p = 0.0030. Each experiment was repeated at least three times utilizing unique primary MSC cultures. *p < 0.05 by Student’s t test