Fig. 4

The effect of BET protein inhibitor on regulation of ACAN, COL2A1, and SOX9 genes in human chondrocytes (a–c). The transcriptional expression (RT-PCR) of ACAN, COL2A1, and SOX9 genes in human chondrocytes, respectively, after the cells were pre-treated with or without I-BET151 (1 μM) followed by addition of IL-1β (10 ng/ml) or TNF-α (10 ng/ml) for 24 h. Relative fold change values were calculated in comparison with vehicle control set as 1 (n = 3). d The protein expression of SOX9 proteins (Western blot) in human chondrocytes treated as in a–c (n = 1)