Table 1 Oligonucleotide primers for PCR amplification
Gene | Primer sequence(5′-3′) | Annealing temperature (°C) |
|---|---|---|
GAPDH | Forward: ACTCTGGCAAAGTGGATG Reverse: TCCTGGAAGATGGTGATG | 60 |
Aggrecan | Forward: GAGGTCGTGGTGAAAGGTGT Reverse: GTGTGGATGGGGTACCTGAC | 62 |
COL1A1 | Forward: AGGGCCAAGACGAAGACATC Reverse: AGATCACGTCATCGCACAACA | 60 |
COL2A1 | Forward: GGATAGACCCCAACCAAGGC Reverse: GCTGCTCCACCAGTTCTTCT | 62 |
MMP-3 | Forward: GCCAAGAGATGCTGTTGATG Reverse: AGGTCTGTGAAGGCGTTGTA | 65 |