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Table 1 Primer pairs used in qPCR

From: Verification of TREX1 as a promising indicator of judging the prognosis of osteosarcoma

Gene Forward primer 5′-3′ Reverse primer 5′-3′ Length
TREX1 5′-CCACTCCTTTCCTTACCACATC-3′ 5′-CCACTCCGCCAAACAGAT-3′ 106 bp
Nanog 5′-ACCTATGCCTGTGATTTGTGGG-3′ 5′-AGAAGTGGGTTGTTTGCCTTTG-3′ 169 bp
Oct4 5′-CTGAGGTGCCTGCCCTTCTA-3′ 5′-CCAACCAGTTGCCCCAAAC-3′ 166 bp
Bglap 5′-CTCACACTCCTCGCCCTATT-3′ 5′-CGCCTGGGTCTCTTCACTAC-3′ 143 bp
adipoQ 5′-CCCATTCGCTTTACCAAGAT-3′ 5′-GGCTGACCTTCACATCCTTC-3′ 139 bp
GADPH 5′-ATGACATCAAGAAGGTGGTG-3′ 5′-CATACCAGGAAATGAGCTTG-3′ 177 bp