Table 1 Primers of collagen I, OCN, and OPN used in RT-PCR in this study
Gene | Primer sequence | |
|---|---|---|
GAPDH | Forward | 5′-ATGGGGAAGGTGAAGGTCG-3′ |
Reverse | 5′-TAAAAGCAGCCCTGGTGACC-3′ | |
Collagen I | Forward | 5′-GGTTTGTTGAAGAGGCTG-3′ |
Reverse | 5′-GATGGCCTGAAGCTCAA-3′ | |
Osteocalcin | Forward | 5′-CCGGGAGGAGATCTGTGAAA-3′ |
Reverse | 5′-CTGCCTTCTTCCACAATTTTATCC-3′ | |
Osteopontin | Forward | 5′-GCCAGTTGCAGCCTTCTCA-3′ |
Reverse | 5′-GCCATGCCCAAGAGACAAAA-3′ |