Adipose-derived mesenchymal stem cells and platelet-rich plasma synergistically ameliorate the surgical-induced osteoarthritis in Beagle dogs

Table 4 Immunohistochemical analysis on the tibia articular cartilages

Cell numbers (cells/mm²)	Sham	Control	MSC	PRP	MP
BrdU+	123.67 ± 24.40	7.00 ± 2.97^h	49.50 ± 12.69^hj	62.00 ± 16.09^hj	120.33 ± 29.27^jkl
IL-1β+	5.33 ± 2.16	170.67 ± 19.94^a	63.50 ± 11.64^ac	41.33 ± 16.10^ac	15.67 ± 10.25^cdf
TNF-α+	6.33 ± 3.14	141.00 ± 32.51^a	70.33 ± 14.17^ac	53.33 ± 15.24^ac	28.50 ± 10.45^bcdg
COX-2+	4.00 ± 2.61	129.67 ± 44.74^h	61.67 ± 13.76^hj	37.50 ± 11.04^hj	18.33 ± 3.93^hjkl
iNOS+	41.17 ± 17.88	243.00 ± 54.32^h	124.33 ± 45.37^hj	73.00 ± 10.97^ij	47.60 ± 13.17^ikl
Caspase-3+	6.00 ± 3.85	102.50 ± 27.06^h	43.17 ± 12.48^hj	25.83 ± 5.53^hj	15.33 ± 4.63^hjk
PARP+	45.67 ± 10.63	134.67 ± 38.53^a	68.33 ± 17.49^c	63.33 ± 19.03^c	39.67 ± 12.36^ceg
IFN-γ+	7.50 ± 2.43	49.67 ± 11.40^h	28.33 ± 5.79^hj	22.33 ± 3.27^hj	13.83 ± 3.82^jkl

Values are expressed as mean ± SD of six dogs
MSC mesenchymal stem cells, PRP platelet-rich plasma, MP MSC and PRP co-treatment
^a p < 0.01 as compared with sham control by LSD test
^b p < 0.05 as compared with sham control by LSD test
^c p < 0.01 as compared with control by LSD test
^d p < 0.01 as compared with MSC treated group by LSD test
^e p < 0.05 as compared with MSC treated group by LSD test
^f p < 0.01 as compared with PRP treated group by LSD test
^g p < 0.05 as compared with PRP treated group by LSD test
^h p < 0.01 as compared with sham control by MW test
ⁱ p < 0.05 as compared with sham control by MW test
^j p < 0.01 as compared with control by MW test
^k p < 0.01 as compared with MSC treated group by MW test
^l p < 0.01 as compared with PRP treated group by MW test

ISSN: 1749-799X