Figure 5

In vitro quantification of transduction efficacy. When determining the efficacy of transduction in human mesenchymal stem cells, we observed an overwhelming (10- to 24-fold) increase of BMP-2 in cell culture supernatants in transfected cells compared to controls. BMP-2 expression increased after the multiplicity of infection (MOI) was doubled from 500 to 1,000 PFU/cell (A). In vivo microscopy of cell cultures 5 days after transduction with Ad.GFP in native ((B) 100×) and UV light ((C) 100×) highlights a successful transduction of human mesenchymal stem cells. A peak in BMP-2 production on the fifth day after transduction was observed when using a MOI of 1,000 PFU/cell and a plateau after the fifth day when MOI 500 was used (D).