Table 1 Primer’s sequences of the targeted genes used for real-time PCR
From: Osmolarity influences chondrocyte repair after injury in human articular cartilage
Target gene | Orientation | Primer sequence (5′-3′) |
|---|---|---|
β-actin | Forward | CACCATTGGCAATGAGCGGTTC |
Reverse | AGGTCTTTG CGGATGTCCACGT | |
HIF-1α | Forward | TATGAGCCAGAAGAACTTTTAGGC |
Reverse | CACCTCTTTTGGCAAGCATCCTG | |
Collagen II | Forward | CCTGGCAAAGATGGTGAGACAG |
Reverse | CCTGGTTTTCCACCTTCACCTG |