Cell attachment and proliferation and ALP activity of monolayer ASC cells. (A) Cell attachment and proliferation on untreated and peroxide-treated demineralized cancellous bone scaffolds. Cell attachment significantly decreased on peroxide-treated scaffolds compared to that on the untreated ones (denoted by asterisk, p < 0.05). However, the cell number was similar for the two groups after 5 days in culture. (B) ALP activity of monolayer ASC cells after 7 days in culture with different demineralized bone powder treatments. Statistically significant differences were found between cultures without powder and cultures supplemented with untreated powder (denoted by asterisk, p < 0.05), as well as cultures with untreated powder and cultures with peroxide-treated powders (denoted by double asterisks, p < 0.05).