Figure 2
From: An ovine in vitro model for chondrocyte-based scaffold-assisted cartilage grafts
Immuno-staining of type II collagen in culture expanded, dedifferentiating ovine articular chondrocytes. Freshly isolated chondrocytes (A) in passage 0 attached to the surface of the cell culture flask and show round-shaped as well as already stretch chondrocytes with strong staining of type II collagen. Further cultivation of the chondrocytes up to passage 1 (B), passage 3 (C) and passage 5 (D) revealed stretched cells of a typical fibroblastic appearance and a gradual loss of type II collagen.