Figure 1

Effect of hypoxia and extracellular Ca²⁺ on cytoplasmic Ca²⁺ levels in bovine articular chondroytes. Chondrocytes were isolated with collagenase at either 20% or 1% O₂ and maintained at these O₂ tensions throughout (16-18 hours). Cytoplasmic Ca²⁺ levels ([Ca²⁺]_i) were then measured with Fura-2 in the presence (2 mM Ca²⁺) or absence (Ca²⁺-free plus 1 mM EGTA) extracellular Ca²⁺. Histograms represent means ± S.E.M., n = 9. * p < 0.02 ** p < 0.006.