Figure 5

The effects of 10% serum, a neutralizing monoclonal antibody and of VEGF receptor-specific ligands on spontaneous Primary Human Osteoblast apoptosis in vitro. Osteoblast apoptosis was determined by Annexin V- Fluorescein Isothiocyanate Labeling and hypodiploid DNA measurement as described in Materials and Methods. mAB VEGF: neutralising monoclonal antibody to vascular endothelial growth factor isotype 165 (0.3 ug/mL), VEGF: vascular endothelial growth factor, PlGF: placental like growth factor. Data illustrates mean +/- standard error of the mean in each case. The results were derived from six separate experiments, with triplicates performed in each experiment. * p < 0.05 represents statistically significant differences compared to control, ^# p < 0.05 represents statistically significant differences compared to 10% serum alone.