A. Representative radiographs taken at day 0, 21 and 35 for the 4 experimental groups. Group1 had shown a gradual improvement in callus formation in radiographic appearance through the time points. The fracture showed sign of union in Group I at day 35 post-fracture. There was no difference between Group II or III compared to Group IV at all the time points. B. Radiographic analysis data at week 5 post-fracture. Group I (100 μg TP508 injected in the fracture gap) had the largest amount of callus across the fracture gap compared to the other groups. Statistical analysis was carried out using non-parametrical Mann Whitney U test, difference between groups was considered significant at *p < 0.05.