Table 1 Primers used in this study

1

TAA TAC GAC TCA CTA TAG GG

T7, amplifying inserts from pCDNA3.1

2

AAG GAC GAT GAT GAC AAA GCT GCA AAT ACA TGT GCA CT

Adding a flag tag

3

TGT CAT CAT CGT CCT TAT AGT CCC CCC AGA CTC CAC AGC T

4

GAG GAG AGG GTT AGG GAT AGG CTT A

Amplifying inserts from pCDNA3.1

5

ACT GCA AGC TGA GCA AGT CTT CTT G

Adding a vWFA domain into minimatn3 (combining with the PCR product of primers 1 and 4)

Figure 4

6

ATCTGC GTT AGA GCC ACA ACA AGC AGT

7

ACT GCA AGC TGA GCA AGT CTT CTT G

Replacing minimatn3 coiled-coil domain with that of matn1 (combining with the PCR product of primers 1 and 4)

Figure 7

8

ATC TGC GTT AGA GCC ACA ACA AGC AGT

9

AAA GAA CAA CCT GGG TGG CAG TCA TGA

Introducing R116W mutation in the vWFA domain

Figure 2

10

TCA TGA CTG CCA CCC AGG TGG TTC TTT

11

GAT GAC AAA GCA CCT CCT CAG CCC AGA

Adding a flag tag

12

ATC TTC CTC ACT GCA GGT CTT CCC ATC ATT

13

ACC TGC AGT GAG GAA GAT CCA TGC GAA TGT

Creating Δmatn1 by deleting vWFA2

Figure 5

14

ACC TGC AGT TGC GAA TGT AAA TCT ATA GT

15

ACA TTC GCA ACT GCA GGT CTT CCC ATC AT

Creating Δmatn1_del by deleting 4 amino acids from Δmatn1

Figure 6

16

ACT TGC TCA GCT TGC AGT GGT GGG TCA

17

TGT GGC TCT AAC GCA GAT TTT CAT TTG

Amplifying mantn1 coiled-coil domain

18

ACT TGC TCA GCT GTC AGT GGT GGG TCA

19

TCT GGC TCT AAC GCA GAT TTT CAT TTG

Amplifying mantn1 vWFA2 domain