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Table 1 Primers used in this study

From: Multiple functions of the von Willebrand Factor A domain in matrilins: secretion, assembly, and proteolysis

Primers Primer Sequences (5'-->3') PCR purpose Results shown in Figures
1 TAA TAC GAC TCA CTA TAG GG T7, amplifying inserts from pCDNA3.1  
2 AAG GAC GAT GAT GAC AAA GCT GCA AAT ACA TGT GCA CT Adding a flag tag  
3 TGT CAT CAT CGT CCT TAT AGT CCC CCC AGA CTC CAC AGC T   
4 GAG GAG AGG GTT AGG GAT AGG CTT A Amplifying inserts from pCDNA3.1  
5 ACT GCA AGC TGA GCA AGT CTT CTT G Adding a vWFA domain into minimatn3 (combining with the PCR product of primers 1 and 4) Figure 4
6 ATCTGC GTT AGA GCC ACA ACA AGC AGT   
7 ACT GCA AGC TGA GCA AGT CTT CTT G Replacing minimatn3 coiled-coil domain with that of matn1 (combining with the PCR product of primers 1 and 4) Figure 7
8 ATC TGC GTT AGA GCC ACA ACA AGC AGT   
9 AAA GAA CAA CCT GGG TGG CAG TCA TGA Introducing R116W mutation in the vWFA domain Figure 2
10 TCA TGA CTG CCA CCC AGG TGG TTC TTT   
11 GAT GAC AAA GCA CCT CCT CAG CCC AGA Adding a flag tag  
12 ATC TTC CTC ACT GCA GGT CTT CCC ATC ATT   
13 ACC TGC AGT GAG GAA GAT CCA TGC GAA TGT Creating Δmatn1 by deleting vWFA2 Figure 5
14 ACC TGC AGT TGC GAA TGT AAA TCT ATA GT   
15 ACA TTC GCA ACT GCA GGT CTT CCC ATC AT Creating Δmatn1_del by deleting 4 amino acids from Δmatn1 Figure 6
16 ACT TGC TCA GCT TGC AGT GGT GGG TCA   
17 TGT GGC TCT AAC GCA GAT TTT CAT TTG Amplifying mantn1 coiled-coil domain  
18 ACT TGC TCA GCT GTC AGT GGT GGG TCA   
19 TCT GGC TCT AAC GCA GAT TTT CAT TTG Amplifying mantn1 vWFA2 domain  
  1. The primers are numbered as in Figure 1.